Composite

Part:BBa_K2201243:Design

Designed by: Svenja Vinke   Group: iGEM17_Bielefeld-CeBiTec   (2017-10-20)


Fusion protein of CFP and YFP with an amber codon in the linker


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 718
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 1308
    Illegal AgeI site found at 1420
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The fusion protein was generated via gibson assembly.


Source

BBa_K592101 and BBa_K404319

References